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验证数据展示

  • IF Staining of HepG2 using CL488-66095

    IF Staining of HepG2 using CL488-66095

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66095 (Lamin B1 antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL488-66095

    FC experiment of HeLa using CL488-66095

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Lamin B1 (CL488-66095, Clone:3C10G12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Lamin B1 Monoclonal antibody

Lamin B1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66095

产品说明书

CloneNo. 3C10G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Lamin B1 Ready-To-Use IHC Kit. Lamin B1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66095-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66095 targets Lamin B1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Lamin B1 fusion protein Ag20522 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lamin B1
别名 LMNB1, LMNB, LMN2, Lamin-B1, laminB1
计算分子量 66 kDa
GenBank蛋白编号BC012295
基因名称 Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
RRIDAB_2883257
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20700
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Lamin B1 antibody CL488-66095Download protocol
FC protocol for CL Plus 488 Lamin B1 antibody CL488-66095Download protocol
Standard Protocols
Click here to view our Standard Protocols