验证数据展示
经过测试的应用
| Positive WB detected in | human kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue, human skin cancer tissue, human colon cancer tissue, human placenta tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60007-1-Ig targets TGFBI / BIGH3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | TGFBI / BIGH3 fusion protein Ag0241 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transforming growth factor, beta-induced, 68kDa |
| 别名 | Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3 |
| 计算分子量 | 683 aa, 75 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC000097 |
| 基因名称 | TGFBI |
| Gene ID (NCBI) | 7045 |
| ENSEMBL Gene ID | ENSG00000120708 |
| RRID | AB_10896828 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | Q15582 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TGFBI / BIGH3 antibody 60007-1-Ig | Download protocol |
| IHC protocol for TGFBI / BIGH3 antibody 60007-1-Ig | Download protocol |
| IF protocol for TGFBI / BIGH3 antibody 60007-1-Ig | Download protocol |
| IP protocol for TGFBI / BIGH3 antibody 60007-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res TGFBI Production by Macrophages Contributes to an Immunosuppressive Microenvironment in Ovarian Cancer. | ||
Cell Rep Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury | ||
Int J Cancer Transforming growth factor-beta-induced protein secreted by peritoneal cells increases the metastatic potential of ovarian cancer cells. | ||
Cell Mol Life Sci TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions. | ||
Front Oncol Identification of Hub Genes Associated With Sensitivity of 5-Fluorouracil Based Chemotherapy for Colorectal Cancer by Integrated Bioinformatics Analysis. | ||
Neoplasia BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation.
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