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验证数据展示

  • WB analysis using 67208-1-Ig

    WB analysis using 67208-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67208-1-Ig (IFIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 67208-1-Ig

    IHC staining of human spleen using 67208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67208-1-Ig (IFIT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFIT3 antibody included
  • IHC staining of human spleen using 67208-1-Ig

    IHC staining of human spleen using 67208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67208-1-Ig (IFIT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFIT3 antibody included
  • IF Staining of HepG2 using 67208-1-Ig

    IF Staining of HepG2 using 67208-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFIT3 antibody (67208-1-Ig, Clone: 2C8B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

IFIT3 Monoclonal antibody

IFIT3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67208-1-Ig

产品说明书

CloneNo. 2C8B10

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SGC-7901 cells, Caco-2 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IFIT3 Ready-To-Use IHC Kit. IFIT3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67208-1-Ig targets IFIT3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 IFIT3 fusion protein Ag7518 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 IFIT3
别名 CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G
计算分子量 56 kDa
观测分子量 60 kDa
GenBank蛋白编号BC004977
基因名称 IFIT3
Gene ID (NCBI) 3437
RRIDAB_2882501
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14879
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.

实验方案

Product Specific Protocols
WB protocol for IFIT3 antibody 67208-1-IgDownload protocol
IHC protocol for IFIT3 antibody 67208-1-IgDownload protocol
IF protocol for IFIT3 antibody 67208-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols