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验证数据展示

  • IF Staining of HepG2 using CL488-67208

    IF Staining of HepG2 using CL488-67208

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 IFIT3 antibody (CL488-67208, Clone: 2C8B10 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-67208

    FC experiment of HeLa using CL488-67208

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IFIT3 (CL488-67208, Clone:2C8B10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated IFIT3 Monoclonal antibody

IFIT3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67208

产品说明书

CloneNo. 2C8B10

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

GARG49, GARG 49, CIG-49, CIG49, CIG 49

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IFIT3 Ready-To-Use IHC Kit. IFIT3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67208-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67208 targets IFIT3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 IFIT3 fusion protein Ag7518 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 IFIT3
别名 GARG49, GARG 49, CIG-49, CIG49, CIG 49
计算分子量 56 kDa
观测分子量 60 kDa
GenBank蛋白编号BC004977
基因名称 IFIT3
Gene ID (NCBI) 3437
RRIDAB_3084326
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14879
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IFIT3 antibody CL488-67208Download protocol
FC protocol for CL Plus 488 IFIT3 antibody CL488-67208Download protocol
Standard Protocols
Click here to view our Standard Protocols