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CoraLite® Plus 488-conjugated IFIT3 Monoclonal antibody

IFIT3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C8B10

Cat no : CL488-67208

Print datasheet

Synonyms

GARG49, GARG 49, CIG-49, CIG49, CIG 49

验证数据展示

  • IF Staining of HepG2 using CL488-67208

    IF Staining of HepG2 using CL488-67208

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 IFIT3 antibody (CL488-67208, Clone: 2C8B10 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-67208

    FC experiment of HeLa using CL488-67208

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IFIT3 (CL488-67208, Clone:2C8B10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IFIT3 Ready-To-Use IHC Kit. IFIT3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67208-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67208 targets IFIT3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen IFIT3 fusion protein Ag7518 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name IFIT3
Synonyms GARG49, GARG 49, CIG-49, CIG49, CIG 49
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC004977
Gene Symbol IFIT3
Gene ID (NCBI) 3437
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14879
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IFIT3 antibody CL488-67208Download protocol
FC protocol for CL Plus 488 IFIT3 antibody CL488-67208Download protocol
Standard Protocols
Click here to view our Standard Protocols