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IFIT3 Monoclonal antibody

IFIT3 Monoclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

2C8B10

Cat No : 67208-1-Ig

Print datasheet

Synonyms

CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G

验证数据展示

  • WB analysis using 67208-1-Ig

    WB analysis using 67208-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67208-1-Ig (IFIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 67208-1-Ig

    IHC staining of human spleen using 67208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67208-1-Ig (IFIT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 67208-1-Ig

    IHC staining of human spleen using 67208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67208-1-Ig (IFIT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67208-1-Ig

    IF Staining of HepG2 using 67208-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFIT3 antibody (67208-1-Ig, Clone: 2C8B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SGC-7901 cells, Caco-2 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67208-1-Ig targets IFIT3 in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity Human
Immunogen IFIT3 fusion protein Ag7518 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name IFIT3
Synonyms CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC004977
Gene Symbol IFIT3
Gene ID (NCBI) 3437
RRIDAB_2882501
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14879
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.

实验方案

Product Specific Protocols
WB protocol for IFIT3 antibody 67208-1-IgDownload protocol
IHC protocol for IFIT3 antibody 67208-1-IgDownload protocol
IF protocol for IFIT3 antibody 67208-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols