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CoraLite®594-conjugated IFIT3 Monoclonal antibody

IFIT3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C8B10

Cat no : CL594-67208

Print datasheet

Synonyms

CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G

验证数据展示

  • IF Staining of HepG2 using CL594-67208

    IF Staining of HepG2 using CL594-67208

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated IFIT3 antibody (CL594-67208, Clone: 2C8B10 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IFIT3 Ready-To-Use IHC Kit. IFIT3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67208-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67208 targets IFIT3 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen IFIT3 fusion protein Ag7518 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name IFIT3
Synonyms CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC004977
Gene Symbol IFIT3
Gene ID (NCBI) 3437
RRIDAB_2920096
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14879
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.

实验方案

Product Specific Protocols
IF protocol for CL594 IFIT3 antibody CL594-67208Download protocol
Standard Protocols
Click here to view our Standard Protocols