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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Aquaporin 4 Polyclonal antibody

Aquaporin 4 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16473

Print datasheet

Synonyms

AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, WCH4

验证数据展示

  • IF Staining of mouse brain using CL488-16473

    IF Staining of mouse brain using CL488-16473

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Aquaporin 4 antibody (CL488-16473) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 16473-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-16473 is Aquaporin 4 Fusion Protein expressed in E. coli.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Aquaporin 4 fusion protein Ag9561 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aquaporin 4
Synonyms AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, WCH4
Calculated Molecular Weight 323 aa, 35 kDa
Observed Molecular Weight 35-37 kDa, 32-34 kDa
GenBank Accession NumberBC022286
Gene Symbol AQP4
Gene ID (NCBI) 361
RRIDAB_2919135
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55087
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Aquaporin 4 antibody CL488-16473Download protocol
Standard Protocols
Click here to view our Standard Protocols