验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
For other applications, we recommend the unconjugated version of this antibody, 16473-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-16473 targets Aquaporin 4 in IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | Aquaporin 4 fusion protein Ag9561 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Aquaporin 4 |
| 别名 | AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, WCH4 |
| 计算分子量 | 323 aa, 35 kDa |
| 观测分子量 | 35-37 kDa, 32-34 kDa |
| GenBank蛋白编号 | BC022286 |
| 基因名称 | Aquaporin 4 |
| Gene ID (NCBI) | 361 |
| RRID | AB_2919135 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55087 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Aquaporin 4 antibody CL488-16473 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |