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CoraLite®555-conjugated BCL2 Monoclonal antibody
BCL2 Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, pig
Applications
IF/ICC, IF-P
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
4H8C6
验证数据展示
经过测试的应用
Positive IF-P detected in | human appendicitis tissue |
Positive IF/ICC detected in | NIH/3T3 cells, MCF-7 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-60178 targets BCL2 in IF/ICC, IF-P applications and shows reactivity with human, mouse, pig samples.
Tested Applications | IF/ICC, IF-P Application Description |
Tested Reactivity | human, mouse, pig |
Immunogen | BCL2 fusion protein Ag3508 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | B-cell CLL/lymphoma 2 |
Synonyms | BCL-2, 4H8C6 |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | BC027258 |
Gene Symbol | BCL2 |
Gene ID (NCBI) | 596 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P10415 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.2) FAQs for Bcl-2
A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL555 BCL2 antibody CL555-60178 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |