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验证数据展示

  • IF Staining of human tonsillitis using CL488-60178

    IF Staining of human tonsillitis using CL488-60178

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:100.

  • IF Staining of human tonsillitis using CL488-60178

    IF Staining of human tonsillitis using CL488-60178

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:100.

  • IF Staining of MCF-7 using CL488-60178

    IF Staining of MCF-7 using CL488-60178

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BCL2 Monoclonal antibody

BCL2 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-60178

Print datasheet

CloneNo. 4H8C6

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC, IF-P

Synonyms

BCL-2, 4H8C6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue, human appendicitis tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60178-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60178 targets BCL2 in IF/ICC, IF-P applications and shows reactivity with human, pig samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, pig
Immunogen BCL2 fusion protein Ag3508 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name B-cell CLL/lymphoma 2
Synonyms BCL-2, 4H8C6
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
RRIDAB_2883111
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BCL2 antibody CL488-60178Download protocol
Standard Protocols
Click here to view our Standard Protocols