验证数据展示
经过测试的应用
Positive IF-P detected in | human tonsillitis tissue, human appendicitis tissue |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-60178 targets BCL2 in IF/ICC, IF-P applications and shows reactivity with human, pig samples.
经测试应用 | IF/ICC, IF-P Application Description |
经测试反应性 | human, pig |
免疫原 | BCL2 fusion protein Ag3508 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | B-cell CLL/lymphoma 2 |
别名 | BCL-2, 4H8C6 |
计算分子量 | 26 kDa |
观测分子量 | 26 kDa |
GenBank蛋白编号 | BC027258 |
基因名称 | BCL2 |
Gene ID (NCBI) | 596 |
RRID | AB_2883111 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P10415 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 BCL2 antibody CL488-60178 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |