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验证数据展示

  • IF Staining of MCF-7 using CL647-60178

    IF Staining of MCF-7 using CL647-60178

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 647 BCL2 antibody (CL647-60178, Clone: 4H8C6 ) at dilution of 1:200.

  • FC experiment of human peripheral blood lymphocytes using CL647-60178

    FC experiment of human peripheral blood lymphocytes using CL647-60178

    1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated BCL2 Monoclonal antibody (CL647-60178, Clone:4H8C6)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2b Isotype Control (MPC-11) (CL647-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated BCL2 Monoclonal antibody

BCL2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-60178

产品说明书

CloneNo. 4H8C6

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

IF/ICC, FC (Intra)

别名

BCL-2, 4H8C6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells, HeLa cells
Positive FC (Intra) detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60178-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-60178 targets BCL2 in IF/ICC, FC (Intra) applications and shows reactivity with human, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, pig
免疫原 BCL2 fusion protein Ag3508 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 B-cell CLL/lymphoma 2
别名 BCL-2, 4H8C6
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC027258
基因名称 BCL2
Gene ID (NCBI) 596
RRIDAB_2934981
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10415
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 BCL2 antibody CL647-60178Download protocol
FC protocol for CL Plus 647 BCL2 antibody CL647-60178Download protocol
Standard Protocols
Click here to view our Standard Protocols