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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated BCL2 Monoclonal antibody

BCL2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4H8C6

Cat no : CL647-60178

Print datasheet

Synonyms

BCL-2, 4H8C6

验证数据展示

  • IF Staining of MCF-7 using CL647-60178

    IF Staining of MCF-7 using CL647-60178

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 647 BCL2 antibody (CL647-60178, Clone: 4H8C6 ) at dilution of 1:200.

  • FC experiment of human peripheral blood lymphocytes using CL647-60178

    FC experiment of human peripheral blood lymphocytes using CL647-60178

    1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated BCL2 Monoclonal antibody (CL647-60178, Clone:4H8C6)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2b Isotype Control (MPC-11) (CL647-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells, HeLa cells
Positive FC (Intra) detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60178-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-60178 targets BCL2 in IF/ICC, FC (Intra) applications and shows reactivity with human, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, pig
Immunogen BCL2 fusion protein Ag3508 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name B-cell CLL/lymphoma 2
Synonyms BCL-2, 4H8C6
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 BCL2 antibody CL647-60178Download protocol
FC protocol for CL Plus 647 BCL2 antibody CL647-60178Download protocol
Standard Protocols
Click here to view our Standard Protocols