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CoraLite® Plus 488-conjugated Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10712

Print datasheet

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

验证数据展示

  • IF Staining of mouse small intestine using CL488-10712

    IF Staining of mouse small intestine using CL488-10712

    Adult mouse small intestine stained for Cytokeratin 19 (green, CL488-10712) and BMI1 (red, 10832-1-AP). BMI1 stains intestinal stem cells located at the base of the villi. As these stem cells differentiate into intestinal epithelial cells, they acquire Cytokeratin 19 expression. Cytokeratin 19 was conjugated to Coralite 488. Samples were fixed in 4% PFA, embedded in paraffin, and imaged on a confocal microscope. Image credit: @Immunofluorescence on Instagram.

  • IF Staining of HeLa using CL488-10712

    IF Staining of HeLa using CL488-10712

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-10712

    FC experiment of MCF-7 using CL488-10712

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Cytokeratin 19 (CL488-10712) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inmouse small intestine tissue, HeLa cells, HepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10712-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10712 targets Cytokeratin 19 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Cytokeratin 19 fusion protein Ag1085 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 19
Synonyms CK19, CK 19, KRT19, K19, Cytokeratin-19
Calculated Molecular Weight 40 kDa
GenBank Accession NumberBC007628
Gene Symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2918994
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08727
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 19 antibody CL488-10712Download protocol
FC protocol for CL Plus 488 Cytokeratin 19 antibody CL488-10712Download protocol
Standard Protocols
Click here to view our Standard Protocols