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验证数据展示

  • IF Staining of mouse small intestine using CL488-10712

    IF Staining of mouse small intestine using CL488-10712

    Adult mouse small intestine stained for Cytokeratin 19 (green, CL488-10712) and BMI1 (red, 10832-1-AP). BMI1 stains intestinal stem cells located at the base of the villi. As these stem cells differentiate into intestinal epithelial cells, they acquire Cytokeratin 19 expression. Cytokeratin 19 was conjugated to Coralite 488. Samples were fixed in 4% PFA, embedded in paraffin, and imaged on a confocal microscope. Image credit: @Immunofluorescence on Instagram.

  • IF Staining of HeLa using CL488-10712

    IF Staining of HeLa using CL488-10712

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-10712

    FC experiment of MCF-7 using CL488-10712

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Cytokeratin 19 (CL488-10712) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for IF, FC (Intra)
Cat No. CL488-10712

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF, FC (Intra)

别名

CK19, CK 19, KRT19, K19, Cytokeratin-19

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF detected inmouse small intestine tissue, HeLa cells, HepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10712-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10712 targets Cytokeratin 19 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Cytokeratin 19 fusion protein Ag1085 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 19
别名 CK19, CK 19, KRT19, K19, Cytokeratin-19
计算分子量 40 kDa
GenBank蛋白编号BC007628
基因名称 Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2918994
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08727
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 19 antibody CL488-10712Download protocol
FC protocol for CL Plus 488 Cytokeratin 19 antibody CL488-10712Download protocol
Standard Protocols
Click here to view our Standard Protocols