CoraLite®594-conjugated Cytokeratin 19 Polyclonal antibody
Cytokeratin 19 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, FC (Intra) and More (1)
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
| Positive FC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10712-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-10712 targets Cytokeratin 19 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | Cytokeratin 19 fusion protein Ag1085 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | keratin 19 |
| Synonyms | Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19 |
| Calculated Molecular Weight | 40 kDa |
| GenBank Accession Number | BC007628 |
| Gene Symbol | Cytokeratin 19 |
| Gene ID (NCBI) | 3880 |
| RRID | AB_2919743 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08727 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Cytokeratin 19 antibody CL594-10712 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
