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验证数据展示

  • IF Staining of HeLa using CL488-20584

    IF Staining of HeLa using CL488-20584

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PI3 Kinase p110 Beta antibody (CL488-20584) at dilution of 1:100.

  • FC experiment of HeLa using CL488-20584

    FC experiment of HeLa using CL488-20584

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PI3 Kinase p110 Beta (CL488-20584) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PI3 Kinase p110 Beta Polyclonal antibody

PI3 Kinase p110 Beta Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-20584

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Synonyms

DKFZp779K1237, p110 BETA, PI3 Kinase p110 Beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 20584-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20584 targets PI3 Kinase p110 Beta in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoinositide-3-kinase, catalytic, beta polypeptide
Synonyms DKFZp779K1237, p110 BETA, PI3 Kinase p110 Beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberNM_006219
Gene Symbol PI3 Kinase p110 Beta
Gene ID (NCBI) 5291
RRIDAB_2919173
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42338
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PI3 Kinase p110 Beta antibody CL488-20584Download protocol
FC protocol for CL Plus 488 PI3 Kinase p110 Beta antibody CL488-20584Download protocol
Standard Protocols
Click here to view our Standard Protocols