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验证数据展示

  • FC experiment of HeLa using CL647-20584

    FC experiment of HeLa using CL647-20584

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PI3 Kinase p110 Beta (CL647-20584) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated PI3 Kinase p110 Beta Polyclonal antibody

PI3 Kinase p110 Beta Polyclonal Antibody for FC (Intra)
Cat No. CL647-20584

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

FC (Intra)

别名

DKFZp779K1237, p110 BETA, PI3 Kinase p110 beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 20584-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-20584 targets PI3 Kinase p110 Beta in FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, catalytic, beta polypeptide
别名 DKFZp779K1237, p110 BETA, PI3 Kinase p110 beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta
计算分子量 123 kDa
观测分子量 110-130 kDa
GenBank蛋白编号NM_006219
基因名称 PI3 Kinase p110 Beta
Gene ID (NCBI) 5291
RRIDAB_2934942
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42338
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 PI3 Kinase p110 Beta antibody CL647-20584Download protocol
Standard Protocols
Click here to view our Standard Protocols