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CoraLite®555-conjugated PI3 Kinase p110 Beta Polyclonal antibody
PI3 Kinase p110 Beta Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
IF/ICC
Conjugate
CoraLite®555 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 20584-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-20584 targets PI3 Kinase p110 Beta in IF/ICC applications and shows reactivity with human, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | phosphoinositide-3-kinase, catalytic, beta polypeptide |
Synonyms | DKFZp779K1237, p110 BETA, PI3 Kinase p110 Beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta |
Calculated Molecular Weight | 123 kDa |
Observed Molecular Weight | 110-130 kDa |
GenBank Accession Number | NM_006219 |
Gene Symbol | PI3 Kinase p110 Beta |
Gene ID (NCBI) | 5291 |
RRID | AB_2919637 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P42338 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.
实验方案
Product Specific Protocols | |
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IF protocol for CL555 PI3 Kinase p110 Beta antibody CL555-20584 | Download protocol |
FC protocol for CL555 PI3 Kinase p110 Beta antibody CL555-20584 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |