验证数据展示
经过测试的应用
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
Biotin-66484 targets human IgM in IHC applications and shows reactivity with Human samples.
经测试应用 | IHC Application Description |
经测试反应性 | Human |
免疫原 | human IgM fusion protein Ag1459 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | immunoglobulin heavy constant mu |
别名 | DKFZp686I15196, DKFZp686I15212, FLJ00385, IGHM, MU, VH |
计算分子量 | 69 kDa |
观测分子量 | 75 kDa |
GenBank蛋白编号 | BC009851 |
基因名称 | IGHM |
Gene ID (NCBI) | 3507 |
RRID | AB_2918947 |
偶联类型 | Biotin |
形式 | Liquid |
纯化方式 | Protein G purification |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).
实验方案
Product Specific Protocols | |
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IHC protocol for Biotin human IgM antibody Biotin-66484 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |