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验证数据展示

  • IHC staining of human tonsillitis using Biotin-66484

    IHC staining of human tonsillitis using Biotin-66484

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66484 (human IgM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgM antibody included

Biotin-conjugated IgM Monoclonal antibody

human IgM Monoclonal Antibody for IHC
Cat No. Biotin-66484

Print datasheet

CloneNo. 2D10B10

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC

Synonyms

DKFZp686I15196, DKFZp686I15212, FLJ00385, IGHM, MU, VH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy human IgM Ready-To-Use IHC Kit. human IgM primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66484 targets human IgM in IHC applications and shows reactivity with Human samples.

Tested Applications IHC Application Description
Tested Reactivity Human
Immunogen human IgM fusion protein Ag1459 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant mu
Synonyms DKFZp686I15196, DKFZp686I15212, FLJ00385, IGHM, MU, VH
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC009851
Gene Symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_2918947
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).

实验方案

Product Specific Protocols
IHC protocol for Biotin human IgM antibody Biotin-66484Download protocol
Standard Protocols
Click here to view our Standard Protocols