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CoraLite® Plus 647-conjugated human IgM Monoclonal antibody

human IgM Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2D10B10

Cat no : CL647-66484

Print datasheet

Synonyms

IgM, IGHM, VH, MU, 2D10B10

验证数据展示

  • IF Staining of human tonsillitis using CL647-66484

    IF Staining of human tonsillitis using CL647-66484

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 human IgM antibody (CL647-66484, Clone: 2D10B10 ) at dilution of 1:100.

  • IF Staining of human appendicitis using CL647-66484

    IF Staining of human appendicitis using CL647-66484

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 647 human IgM antibody (CL647-66484, Clone: 2D10B10 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman appendicitis tissue, human tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy human IgM Ready-To-Use IHC Kit. human IgM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66484-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66484 targets human IgM in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen human IgM fusion protein Ag1459 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant mu
Synonyms IgM, IGHM, VH, MU, 2D10B10
Calculated Molecular Weight 69 kDa
GenBank Accession NumberBC009851
Gene Symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_2920286
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 human IgM antibody CL647-66484Download protocol
Standard Protocols
Click here to view our Standard Protocols