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HRP-conjugated human IgM Monoclonal antibody

human IgM Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

HRP

CloneNo.

2D10B10

Cat no : HRP-66484

Print datasheet

Synonyms

DKFZp686I15196, DKFZp686I15212, FLJ00385, human IgM, IGHM, MU, VH

验证数据展示

  • WB analysis using HRP-66484

    WB analysis using HRP-66484

    0.1 μL human plasma was subjected to SDS PAGE followed by western blot with HRP-66484 (HRP conjuaged mouse anti-human IgM) at dilution of 1:10000, 1:20000, 1:40000, incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma
Planning an IHC experiment? We recommend our IHCeasy human IgM Ready-To-Use IHC Kit. human IgM primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

HRP-66484 targets human IgM in WB applications and shows reactivity with Human samples.

Tested Applications WB Application Description
Cited ApplicationsWB
Tested Reactivity Human
Immunogen human IgM fusion protein Ag1459 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant mu
Synonyms DKFZp686I15196, DKFZp686I15212, FLJ00385, human IgM, IGHM, MU, VH
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC009851
Gene Symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_2883841
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the heavy chain of human IgM (~75 kDa). It's HRP conjugated.

实验方案

Product Specific Protocols
WB protocol for HRP human IgM antibody HRP-66484Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Mol Ther

Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transfer

Authors - Timothy J Smith
View Article