WB result of ICAM1 antibody (10831-1-AP, 1:1000) with si-Control and si-ICAM1 transfected Raji cells..
Various lysates were subjected to SDS PAGE followed by western blot with 10831-1-AP (ICAM-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10831-1-AP (ICAM-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10831-1-AP (ICAM-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using ICAM-1 antibody (10831-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using ICAM-1 antibody (10831-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP Result of anti-ICAM-1 (IP:10831-1-AP, 4ug; Detection:10831-1-AP 1:1000) with Raji cells lysate 1320ug.
1X10^6 Raji cells were stained with 0.2ug ICAM-1 antibody (10831-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.