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Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G1E4

Cat No : 60187-1-Ig

Print datasheet

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19



经过测试的应用

Positive WB detected inMCF-7 cells, HaCaT cells, PC-3 cells, HeLa cells, T-47D cells, pig colon tissue, rat colon tissue, mouse colon tissue
Positive IHC detected inhuman colon tissue, human thyroid cancer tissue, human stomach cancer tissue, human lung cancer tissue, human breast cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:1000-1:51200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60187-1-Ig targets Cytokeratin 19 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Immunogen Cytokeratin 19 fusion protein Ag7407 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name keratin 19
Synonyms Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC002539
Gene Symbol KRT19
Gene ID (NCBI) 3880
RRIDAB_10859834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08727
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 60187-1-IgDownload protocol
IHC protocol for Cytokeratin 19 antibody 60187-1-IgDownload protocol
IF protocol for Cytokeratin 19 antibody 60187-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy

Authors - Xiaoyu Liu
humanWB

Stem Cells

Annexin A3 as a Potential Target for Immunotherapy of Liver Cancer Stem-Like Cells.

Authors - Qiu-Zhong Pan
humanIF

Breast Cancer Res

Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors

Authors - Wei Yang
mouseIF

Biomed Pharmacother

Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis.

Authors - Xufeng Tao
mouseIF

Biochim Biophys Acta Mol Basis Dis

TGF-β/YB-1/Atg7 axis promotes the proliferation of hepatic progenitor cells and liver fibrogenesis

Authors - Yuecheng Guo
humanIHC

Reprod Sci

Identification of Key Differentially Methylated/Expressed Genes and Pathways for Ovarian Endometriosis by Bioinformatics Analysis

Authors - Haibo Zhang