验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HaCaT cells, PC-3 cells, HeLa cells, T-47D cells, pig colon tissue, rat colon tissue, mouse colon tissue |
| Positive IHC detected in | human colon tissue, human breast cancer tissue, human lung cancer tissue, human oesophagus cancer tissue, human stomach cancer tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:51200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60187-1-Ig targets Cytokeratin 19 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, FC (Intra) |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human |
| 免疫原 | Cytokeratin 19 fusion protein Ag7407 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | keratin 19 |
| 别名 | CK19, CK 19, KRT19, Cytokeratin-19, Cytokeratin,Keratin |
| 计算分子量 | 44 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC002539 |
| 基因名称 | Cytokeratin 19 |
| Gene ID (NCBI) | 3880 |
| RRID | AB_10859834 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08727 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| IF protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy | ||
Stem Cells Annexin A3 as a Potential Target for Immunotherapy of Liver Cancer Stem-Like Cells. | ||
Stem Cell Res Ther DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis | ||
Breast Cancer Res Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors | ||
Commun Biol Chemerin attenuates acute kidney injury by inhibiting ferroptosis via the AMPK/NRF2/SLC7A11 axis | ||
Biomed Pharmacother Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis. |
