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Cytokeratin 19 Monoclonal antibody
Cytokeratin 19 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
WB, IHC, IF-P, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3G1E4
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HaCaT cells, PC-3 cells, HeLa cells, T-47D cells, pig colon tissue, rat colon tissue, mouse colon tissue |
| Positive IHC detected in | human colon tissue, human thyroid cancer tissue, human stomach cancer tissue, human lung cancer tissue, human breast cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:51200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60187-1-Ig targets Cytokeratin 19 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.
| Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC, IF, FC (Intra) |
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Immunogen | Cytokeratin 19 fusion protein Ag7407 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | keratin 19 |
| Synonyms | Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC002539 |
| Gene Symbol | Cytokeratin 19 |
| Gene ID (NCBI) | 3880 |
| RRID | AB_10859834 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08727 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| IF protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| FC protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy | ||
Stem Cells Annexin A3 as a Potential Target for Immunotherapy of Liver Cancer Stem-Like Cells. | ||
Stem Cell Res Ther DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis | ||
Breast Cancer Res Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors | ||
Biomed Pharmacother Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis. | ||
Biochim Biophys Acta Mol Basis Dis TGF-β/YB-1/Atg7 axis promotes the proliferation of hepatic progenitor cells and liver fibrogenesis |
