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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3G1E4

Cat no : CL647-60187

Print datasheet

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

验证数据展示

  • IF Staining of human oesophagus cancer using CL647-60187

    IF Staining of human oesophagus cancer using CL647-60187

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • IF Staining of human breast cancer using CL647-60187

    IF Staining of human breast cancer using CL647-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • IF Staining of human breast cancer using CL647-60187

    IF Staining of human breast cancer using CL647-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL647-60187

    FC experiment of HeLa using CL647-60187

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Cytokeratin 19 (CL647-60187, Clone:3G1E4) (red), or 0.8 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inhuman breast cancer tissue, human oesophagus cancer tissue
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60187-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-60187 targets Cytokeratin 19 in IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human
Immunogen Cytokeratin 19 fusion protein Ag7407 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name keratin 19
Synonyms Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC002539
Gene Symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2920244
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08727
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Cytokeratin 19 antibody CL647-60187Download protocol
FC protocol for CL Plus 647 Cytokeratin 19 antibody CL647-60187Download protocol
Standard Protocols
Click here to view our Standard Protocols