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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G1E4

Cat no : CL488-60187

Print datasheet

Synonyms

CK19, CK 19, KRT19, Cytokeratin-19, Cytokeratin,Keratin

验证数据展示

  • IF Staining of HepG2 using CL488-60187

    IF Staining of HepG2 using CL488-60187

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-60187 (Cytokeratin 19 antibody) at dilution of 1:50.

  • FC experiment of HeLa using CL488-60187

    FC experiment of HeLa using CL488-60187

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Cytokeratin 19 (CL488-60187, Clone:3G1E4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human breast cancer using CL488-60187

    IF Staining of human breast cancer using CL488-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-60187, Clone: 3G1E4 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60187-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60187 targets Cytokeratin 19 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human
Immunogen Cytokeratin 19 fusion protein Ag7407 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name keratin 19
Synonyms CK19, CK 19, KRT19, Cytokeratin-19, Cytokeratin,Keratin
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC002539
Gene Symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2883114
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08727
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 19 antibody CL488-60187Download protocol
FC protocol for CL Plus 488 Cytokeratin 19 antibody CL488-60187Download protocol
Standard Protocols
Click here to view our Standard Protocols