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验证数据展示

  • IF Staining of human breast cancer using CL555-60187

    IF Staining of human breast cancer using CL555-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • IF Staining of human breast cancer using CL555-60187

    IF Staining of human breast cancer using CL555-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-60187

    FC experiment of HeLa using CL555-60187

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 19 (CL555-60187, Clone:3G1E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL555-60187

Print datasheet

CloneNo. 3G1E4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P, FC (Intra)

Synonyms

CK19, CK 19, KRT19, Cytokeratin-19, Cytokeratin,Keratin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman breast cancer tissue, human oesophagus cancer tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60187-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-60187 targets Cytokeratin 19 in IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human
Immunogen Cytokeratin 19 fusion protein Ag7407 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name keratin 19
Synonyms CK19, CK 19, KRT19, Cytokeratin-19, Cytokeratin,Keratin
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC002539
Gene Symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2919658
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08727
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

实验方案

Product Specific Protocols
IF protocol for CL555 Cytokeratin 19 antibody CL555-60187Download protocol
FC protocol for CL555 Cytokeratin 19 antibody CL555-60187Download protocol
Standard Protocols
Click here to view our Standard Protocols