1x10^6 HEK-293 cells (dash lines) and DYKDDDDK-TDP43 Transfected HEK-293 cells (solid lines) were intracellularly stained with 0.2 μg DYKDDDDK tag Monoclonal antibody (66008-4-Ig, Clone: 8H6A10, red) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Mouse IgG2b isotype control (66360-3-Ig, Clone: 11B8C4, blue) was parallel stained as control. Cells were fixed with 4% PFA .

HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control.

Immunofluorescent analysis of (-20°C Ethanol) fixed DYKDDDDK-TDP43 Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

IP result of anti-DYKDDDDK tag (IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug.

1x10^6 HEK-293 cells (dash lines) and DYKDDDDK-PCSK9 Transfected HEK-293 cells (solid lines) were intracellularly stained with 0.2 μg DYKDDDDK tag Monoclonal antibody (66008-4-Ig, Clone: 8H6A10, red) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Mouse IgG2b isotype control (66360-3-Ig, Clone: 11B8C4, blue) was parallel stained as control. Cells were fixed with 4% PFA .

IP result of anti-DYKDDDDK tag (IP:80010-1-RR, 2.5 ug; WB:66008-3-Ig, 1:3000) with Transfect HEK-293 cells lysate 768 ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 (eGFP-DYKDDDDK transfected) cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), eGFP (Green), DYKDDDDK (Red), DAPI (Blue).

HEK-293T cells and transfected HEK-293T lysates were subjected to SDS PAGE followed by western blot with 20543-1-AP (DYKDDDDK tag antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

1x10^6 HEK-293 cells (dash lines) and DYKDDDDK-TDP43 Transfected HEK-293 cells (solid lines) were intracellularly stained with 0.2 μg DYKDDDDK tag Monoclonal antibody (66008-4-Ig, Clone: 8H6A10, red) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Mouse IgG2b isotype control (66360-3-Ig, Clone: 11B8C4, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed DYKDDDDK-TDP43 Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

IP result of anti-DYKDDDDK tag (IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

1x10^6 HEK-293 cells (dash lines) and DYKDDDDK-PCSK9 Transfected HEK-293 cells (solid lines) were intracellularly stained with 0.2 μg DYKDDDDK tag Monoclonal antibody (66008-4-Ig, Clone: 8H6A10, red) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Mouse IgG2b isotype control (66360-3-Ig, Clone: 11B8C4, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite®488-conjugated version of this antibody, CL488-66008-3 (DDDDK tag antibody), at dilution of 1:100.

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-66008, Clone: 8H6A10 ) at dilution of 1:200.

Ag2329 (DYKDDDDK tagged protein) was coated onto microtiter plates at 0.073 µg/well and then incubated with a dilution series of Biotin-66008-4 (start concentration 500 ng/ml). And then detected with Streptavidin Poly-HRP (1:2000) followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm. Negative control is 66008-4-Ig.

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using Biotin DYKDDDDK tag antibody (Biotin-66008-4, Clone: 8H6A10 ) at dilution of 1:600 and Alexa Fluor 488 Streptavidin.

Various lysates were subjected to SDS PAGE followed by western blot with CL750-80010 (DYKDDDDK tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.